The first antioxidant enzyme to be identified and studied was catalase (EC 18.104.22.168). Catalases is a crucial enzyme in preventing reactive oxygen species from causing oxidative damage to the cell (ROS). It can catalyse the oxidation of many metabolites and poisons, including as formaldehyde, formic acid, phenols, acetaldehyde, and alcohols, by hydrogen peroxide. Catalase in our bodies converts dangerous superoxide radicals into hydrogen peroxide, which then decomposes into water and oxygen. Microbiologists frequently utilise the catalase test to identify and distinguish between various bacterial species. The food sector uses catalase frequently. By removing hydrogen peroxide from milk, catalase helps with the creation of cheese. Catalase is utilised in food packaging because it stops food from oxidising. In contact lenses, catalase is utilised as a disinfectant. Catalase is used in the elimination of hydrogen peroxide residues from cotton fabrics which improve the dyeing behavior and the colour yield. Kinetics of enzyme catalyzed reaction with respect to aims of enzyme kinetic studies, steady state and transient state kinetics, enzyme activity measurements, factors affecting enzyme activity, experimentally determined rate parameters for Michelis-Menten type kinetics, selective and potent inhibition kinetics, quantitative enzyme characterization: effect of pH, temperature, ionic strength, concentration and enzyme specificity will be discussed. Provide an understanding of role of an enzyme in metabolic pathways.
Dr. Meera Yadav completed her Master’s and Ph.D in Chemistry from DDU Gorakhpur University, Gorakhpur, Uttar Pradesh, India. She was awarded CSIR-SRF (2000-2003), twice as DST- Woman scientist A (2005-2008) & (2013-2016) and UGC- Dr. D. S Kothari Postdoctoral Fellow (2009-2012) at Department of Chemistry, Gorakhpur University, Gorakhpur. Her research area includes, purification, characterization and applications of Metalloenzymes- Lignin peroxidase, Mn-peroxidase, Laccase and Chloroperoxidase, isolation of fungal strains producing ligninolytic enzymes, their structural-functional studies using X-ray crystallography, spectroscopic and fast reaction kinetic techniques. Use of enzymes in synthetic inorganic and organic chemistry. She has served two years as guest lecturer in Madan Mohan Malviya Technical University (MMMTU), Gorakhpur. Currently she is working as an Assistant professor in the Department of Chemistry, North Eastern Regional Institute of Science and Technology (NERIST), Nirjuli, Itanagar, Arunachal Pradesh, India. She has attended and participated in more than 30 national and international seminars and conferences. She has guided one Ph.D and five students are being involved in various research activities in her laboratory. Eight students have submitted their M.Sc dissertation under her guidance till now. She has published more than 40 research papers in reputed national and international peer reviewed journals and reputed standard books. She has also reviewed many research papers. She is life member of American Chemical Society, Indian Council of Chemists and Indian Society of Chemists and Biologists.