Title : Immobilization of bacterial maltase using different polymers: An Excellent design of using maltase in continuous industrial process
The current study deals with the comparative study related to immobilization of maltase using synthetic (polyacrylamide) and non-synthetic (calcium alginate, agar-agar and agarose) polymers via entrapment technique. Polyacrylamide beads were formed by cross-linking of monomers, agar-agar and agarose through solidification while alginate beads were prepared by simple gelation. Results showed that the efficiency of enzyme significantly improved after immobilization and among all tested supports agar-agar was found to be the most promising and biocompatible for maltase in terms of immobilization yield (82.77%). The catalytic behavior of maltase was slightly shifted in terms of reaction time (free enzyme, agarose and polyacrylamide: 05 minutes; agar-agar and alginate: 10 minutes), pH (free enzyme, alginate and polyacrylamide: 6.5; agar-agar, agarose: 7.0) and temperature (free enzyme: 45°C; alginate: 50°C; polyacrylamide: 55°C; agarose: 60°C; agar-agar: 65°C). Furthermore, evaluation of kinetic study revealed that after entrapment the affinity (Km) and maximum velocity (Vmax) of maltase was affected. Activation energy of entrapped maltase decreased which increases its stability at higher temperatures for longer time period. Stability profile of immobilized maltase also revealed that all the supports utilized have significantly enhanced the activity of maltase at higher temperatures then its free counterpart. However, recycling data showed that agar-agar entrapped maltase retained 20% of its initial activity even after 10 cycles followed by agarose (10%) while polyacrylamide and alginate showed no activity after 8 and 6 cycles respectively. Thus, it can be anticipated from current study that immobilization of maltase using various supports effectively improve the catalytic properties of enzyme for its efficient utilization in different bioprocesses.
- The audience will be able to use immobilization technology in industrial process. I will discuss enzyme engineering with the latest challenges and will provide proficient skills and entrepreneurship opportunities in the field of Enzyme Biotechnology. My talk will discuss to establish more linkage programs and collaboration for improving competitive skills and learning horizon of researchers.