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Rasha Mohamed E N, Speaker at Speaker for Catalysis Conference- Rasha Mohamed El Nashar
Cairo University, Egypt
Title : Design and application of virus imprinted polymer (VIP) for the rapid and selective detection of Foot and Mouth Disease Virus (FMDV)

Abstract:

Foot and mouth disease virus (FMDV), is a highly contagious virus due to its ease of transmission. FMDV has seven genetically distinguished serotypes with many subtypes within each serotype. The traditional diagnostic methods of FMDV have demonstrated many drawbacks related to sensitivity, specificity, and cross-reactivity. In the current study, a new viral imprinted polymer (VIP)-based biosensor was designed and fabricated for the rapid and selective detection of the FMDV. The bio-recognition components were formed via electrochemical polymerization of the oxidized O-aminophenol (O-AP) film imprinted with FMDV serotype O on a gold screen-printed electrode (SPE). The overall changes in the design template have been investigated using cyclic voltammetry (CV), atomic force microscopy (AFM), Field emission-scanning electron microscopy (FE-SEM), and Fourier-transform infrared spectroscopy (FT-IR). Optimal conditions were achieved through investigating the capturing efficiency, binding stability, selectivity and life-time of the developed biosensor. The results depicted a high selectivity of the biosensor to the serotype O over all other genus serotypes A, SAT2 and Lumpy skin disease virus (LSDV), as well as, the inactivated serotype O. The limits of detection (LOD) and quantification (LOQ) were around 2 ng/mL and 6 ng/mL, respectively, in addition to the tested repeatability and reproducibility values with a variance coefficient of 1.0% and 3.6%, respectively. In comparison with the reference methods (ELISA and PCR), the analysis of saliva real samples using the developed affordable biosensor offered 50 folds lower LOD with the possibility of an on-line monitoring in the field with no prior sample treatment.  

Biography:

She is full Professor of Analytical Chemistry, Faculty of Science, Cairo University since 2014; adjunct professor in Zewail City of Science and Technology, Giza. She has got the State incentive award for technological science (basic sciences sector)for the year 2015, besides several travel and research grants from DAAD, TWAS and Center of special studies in Alexandria library.  Her research interests include: Molecularly imprinted polymers synthesis and different applications including solid phase extraction, sensors and drug delivery systems, nanoparticles synthesis. She has published more than 45 research articles in SCI(E) journals.

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