Analysis of phosphoric ore bacterial and eucaryal microbial diversity by molecular tools and small-subunit (SSU) sequencing

Title : Analysis of phosphoric ore bacterial and eucaryal microbial diversity by molecular tools and small-subunit (SSU) sequencing

Abstract:

Pollution of water and soils with heavy metals is becoming one of the most severe environmental and human health hazards. Therefore, the application of heavy metal-solubilizing microorganisms is a promising approach for increasing heavy metal bioavailability in heavy metal amended soils. The living communities that exist in extreme conditions have always attracted much interest from taxonomists, microbiologists, and ecologists alike. Little data was found about the microbial structure of mining residue from phosphoric ore processing and the level of cultivable microorganisms reported on these habitats is extremely low. In order to better understand the functions of the microbial community, a full description of the microbial ecosystem previously unknown is required. Recent progress in the Molecular biological tools of microorganisms for industrial purposes promises to revitalize not only the bacterial leaching of metal-bearing ores but also the microbiological treatment of metal-contaminated waste water. The aim of this study was to investigate the phosphoric ore bacterial and eucaryal microbial diversity by by using culture-dependent molecular approaches.According to the highly bacterial variable V3 region of 16S rRNA sequences, bacterial species were identified as :  Stenotrophomonas maltophilia , Stenotrophomonas sp  and Halomicronema hongdechloris as a predominant bacteria in the miming residue. The phylogenetic analysis revealed  Aphanomyces astaci  Neurospora crassa  Phytophthora sojae  as the most common eucaryal taxa for the different sites of interest. These findings provide new opportunities into phosphoric ore microbiology that could be useful in for the bioremediation of heavy metal contaminated sewage and waste water.

Audience Takeaway Notes:

• The use of these bacteria and eucaryal species for the removal of metal cations can also be an interesting alternative.
• The application of heavy metal-solubilizing microorganisms is a promising approach for increasing heavy metal bioavailability in heavy metal amended soils.
• Better understand the functions of the microbial community in mining environment.
• A full description of the microbial ecosystem previously unknown is available.

Biography:

Ilhem OMRI is a Doctor engineer from National Institutes Of Applied Sciences and Technology of Tunisia (INSAT). She is currently Municipal advisor in the City of GAFSA Tunisia. She is in charge of Health, safety and Environment committee. Dr Ilhem OMRI received her PhD in 2013 from the University of Carthage In Tunisia where she has successfully completed her research work on “Bioprocess for H2S removal from waste gas " at Laboratory of Microbial Ecology and Technology at National Institute of Applied Sciences and Technology and then held a Postdoctoral Fellowship at  Campagny of phosphates of GAFSA. She got very good experience in designing bioreactors. She has 12 years of teaching and research experience in Microbiol process and Environmental engineering. Her research experience is mainly on Applied and environmental microbiology, Bioremediation, Bioprocess using Bioreactors, Biofilters, various Biological treatment methods for waste gas and wastewater the microbial diversity of various naturals’ ecosystems using molecular tools.  She has handled more than 5 research projects funded by various companies. More than 20 students have awarded PhD and engineer degrees under her guidance. Dr Ilhem OMRI  has published  papers in journals with high impact factor.