Speaker at Catalysis conferences 2021 - Wojciech Snoch
Wojciech Snoch
Polish Academy of Sciences, Poland
Title : Biocatalysis in search of effective anticancer agents – A novel sugar esters based on polyhydroxyalkanoate monomers

Abstract:

Bacterial polyhydroxyalkanoates (PHA) are natural polyesters synthetized in unfavorable environmental conditions to store and reuse carbon source inside a microbial cell.  They are also a rich source of valuable intermediates that can be chemically modified in numerous ways and used in biocatalytic reactions. This is possible due to the 3-R-hydroxyl and carboxyl groups of the PHA monomers. One example of such use is a synthesis of fatty acid sugar esters (SFAE) catalyzed by fungal immobilized lipases (e.g. Canadida anctrctica lipase B - CalB, Thermomyces lanuginosa) in anhydrous conditions. Since the 1970s, scientists have demonstrated anticancer properties of SFAE  and continuously investigate how modifications of the hydrophobic component affect cancer cells and enhance their therapeutic potential. However, no other group used PHA monomers as such component so far.

During the presentation I will bring the audience closer not only to cancer statistics but also need to search for novel anticancer agents and therapies. A process of synthesis, obtainment and manufacturing of PHA polymers in order to produce hydroxyacids will also be described. I will show possibility of introducing trifluoroethyl groups into PHA monomer molecules selectively. Further, I will explain how these modified and unmodified PHA monomers are used for the synthesis of sugar esters using immobilized lipases, and what molecules have cytotoxic properties against Du145 prostate cancer cells and HTB140 skin melanoma. I will discuss why these molecules are interesting in search of new anti-cancer therapies and their prospective application to enhance the effectiveness of other therapies.

Acknowledgements: This work was supported by The National Science CentrePoland [SONATA no. 2015/17/D/ST4/00514] and InterDokMed project no. POWR.03.02.00-00-I013/16

Audience take-away:

  • The audience will learn how to perform fed-batch cultivation leading to bacterial polyhydroxyalkanoates and produce monomers in order to obtain valuable synthons for biocatalysis.
  • Listeners will find out what fungal lipases one can use to synthetize sugar fatty acid esters based on polyhydroxyalcanoates monomers.
  • Audience will know how one can selectively introduce fluoroethyl moieties into hydroxyacid molecules using free 3-R hydroxyl group and which parameters, conditions are crucial to get desired products.
  • Why one can consider sugar fatty acid esters as anticancer agents and what is their potential to support commonly used anticancer drugs.
  • The participants will be able to expand their knowledge about antiproliferative compounds.
  • Knowing conditions of my reactions they will be able to perform reaction of esterification using their molecules which possess –OH and -COOH groups
  • The audience will be able to apply newly described way of protection 3-R-OH group of hydroxyacids to try different types of modification in their laboratories.

Biography:

Wojciech Snoch studied biology at Jagiellonian University, Poland, graduated as MS in 2012. After the graduation he became a laboratory worker at Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences (PAS) for next eight months. In 2013 he was organizing a new laboratory at Faculty of Chemical Engineering and Technology of Cracow University of Technology. Then in 2015-16 he worked as a biotechnologist at Petroster- a company specialized in bioremediation of soil using fungal and bacterial consortia. Since 2016 he is doing doctoral thesis under dr Guzik and Prof Szaleniec at PAS.

Watsapp